Human APOE2/Apolipoprotein E Protein (APO-HM1E2)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 27.11 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Lys19-His317(R176C)
Catalog #APO-HM1E2
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Product Description
 Recombinant Human APOE2/Apolipoprotein E Protein is expressed from HEK293 with His tag at the C-terminus.It contains Lys19-His317(R176C).
Product Background
 Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
Product Category
 Recombinant Protein
Target
 APOE2/Apolipoprotein E
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys19-His317(R176C)
Molecular Weight
 The protein has a predicted MW of 35.28 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 27.11 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE2, His Tag with an affinity constant of 27.11 nM as determined in SPR assay (Biacore T200).
Human APOE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human APOE2 is greater than 95% as determined by SEC-HPLC.

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