Human APOH Protein (APH-HM101)
Human APOH Protein (APH-HM101)
Human APOH Protein (APH-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human APOH, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly20-Cys345
Catalog #APH-HM101
Regular price $264.00
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Product Description

Recombinant Human APOH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys345.

Product Background

Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

APOH

Synonyms

Apo-H; B2GP1; B2G1; APOH; B2GPI; beta(2)GPI; BG;β(2)GPI;

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly20-Cys345

Molecular Weight

The protein has a predicted MW of 37.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human APOH, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human APOH, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. Immobilized Human APOH, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA.
Human APOH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human APOH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human APOH is greater than 95% as determined by SEC-HPLC. The purity of Human APOH is greater than 95% as determined by SEC-HPLC.

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