Product Description |
Recombinant Human APOH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys345. |
Product Background |
Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele. |
Product Category |
Recombinant Protein |
Target |
APOH |
Synonyms |
Apo-H; B2GP1; B2G1; APOH; B2GPI; beta(2)GPI; BG;β(2)GPI; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly20-Cys345 |
Molecular Weight |
The protein has a predicted MW of 37.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human APOH, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human APOH, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. |
|
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Human APOH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human APOH is greater than 95% as determined by SEC-HPLC. |
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