Human APOH Protein (APH-HM201)

Biotinylated Anti-APOH Antibody, hFc-Avi Tag captured on CM5 Chip via Streptavidin can bind Human APOH, hFc Tag with an affinity constant of 11.10 nM as determined in SPR assay (Biacore T200).

Human APOH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human APOH is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 11.10 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly20-Cys345

Catalog #APH-HM201

Price

$345⁰⁰

Size

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Human APOH Protein (APH-HM201) Summary

Product Description

Recombinant Human APOH Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Gly20-Cys345.

Product Background

Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele.

Human APOH Protein (APH-HM201) Specifications/Details

Protein	APOH
Synonyms	Apo-H; B2GP1; B2G1; APOH; B2GPI; beta(2)GPI; BG;β(2)GPI;;b(2)GPI
Accession	P02749
Species	Human
Biotinylated	no
Amino Acid Range	Gly20-Cys345
Molecular Weight	The protein has a predicted MW of 62.20 kDa. Due to glycosylation, the protein migrates to 80-95 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 11.10 nM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Anti-APOH Antibody, hFc-Avi Tag captured on CM5 Chip via Streptavidin can bind Human APOH, hFc Tag with an affinity constant of 11.10 nM as determined in SPR assay (Biacore T200).
	Human APOH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human APOH is greater than 95% as determined by SEC-HPLC.