Human APRIL/TNFSF13 Protein (active trimer) (APR-HM113)

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human APRIL, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag (Cat. BCM-HM217) with the EC50 of 1.2ng/ml determined by ELISA.
Amino Acid Range: Ala105-Leu250

Catalog #APR-HM113

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$1,050.00

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Human APRIL/TNFSF13 Protein (active trimer) (APR-HM113) Summary

Product Description	Recombinant Human APRIL/TNFSF13 Protein is expressed from HEK293 with His tag and Flag tag at the N-terminus.It contains Ala105-Leu250.
Product Background	The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Protein	APRIL/TNFSF13
Synonyms	CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
Accession	O75888-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala105-Leu250
Molecular Weight	The protein has a predicted MW of 18.16 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human APRIL, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag (Cat. BCM-HM217) with the EC50 of 1.2ng/ml determined by ELISA.
Product Tag	N-His-Flag
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human APRIL, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag (Cat. BCM-HM217) with the EC50 of 1.2ng/ml determined by ELISA.
	Immobilized Human APRIL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag (Cat. TAC-HM213) with the EC50 of 21.1ng/ml determined by ELISA.
	Immobilized Human APRIL, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APRIL Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
	Human APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human APRIL is greater than 95% and the molecular weight of this protein is around 55-70 kDa as determined by SEC-MALS.
	The purity of Human APRIL is greater than 95% as determined by SEC-HPLC.

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