Product Description |
Recombinant Human-AREG-Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Ser101-Lys187. |
Product Background |
Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF‑κB signalling pathway in pancreatic cancer cells. |
Product Category |
Recombinant Protein |
Target |
AREG |
Synonyms |
Amphiregulin; AR; SDGF; AREG; AREGB; CRDGF; MGC13647 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser101-Lys187 |
Molecular Weight |
The protein has a predicted MW of 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 32.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 32.5ng/ml determined by ELISA (QC Test). |
|
|
Human-AREG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human-AREG is greater than 95% as determined by SEC-HPLC. |
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