Name: Human-AREG-Protein (AEG-HM201)
Brand: KactusBio
SKU: AEG-HM201-100UG
Price: 300.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 32.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ser101-Lys187

Catalog #AEG-HM201

Size

100UG

500UG

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Product Description	Recombinant Human-AREG-Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Ser101-Lys187.
Product Background	Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF‑κB signalling pathway in pancreatic cancer cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	AREG
Synonyms	Amphiregulin; AR; SDGF; AREG; AREGB; CRDGF; MGC13647
Accession	P15514
Species	Human
Biotinylated	no
Amino Acid Range	Ser101-Lys187
Molecular Weight	The protein has a predicted MW of 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 32.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 32.5ng/ml determined by ELISA (QC Test).
	Human-AREG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human-AREG is greater than 95% as determined by SEC-HPLC.