Human-AREG-Protein (AEG-HM201)
Human-AREG-Protein (AEG-HM201)
Human-AREG-Protein (AEG-HM201)
Human-AREG-Protein (AEG-HM201)
Human-AREG-Protein (AEG-HM201)
Human-AREG-Protein (AEG-HM201)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser101-Lys187
Catalog #AEG-HM201
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Product Description
 Recombinant Human-AREG-Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Ser101-Lys187.
Product Background
 Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF‑κB signalling pathway in pancreatic cancer cells.
Product Category
 Recombinant Protein
Target
 AREG
Synonyms
 Amphiregulin; AR; SDGF; AREG; AREGB; CRDGF; MGC13647
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser101-Lys187
Molecular Weight
 The protein has a predicted MW of 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA.
Human-AREG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human-AREG is greater than 95% as determined by SEC-HPLC.
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