Human-AREG-Protein (AEG-HM201)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser101-Lys187

Catalog: AEG-HM201

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Product Description	Recombinant Human-AREG-Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Ser101-Lys187.
Product Background	Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF‑κB signalling pathway in pancreatic cancer cells.
Product Category	Recombinant Protein

Target	AREG
Synonyms	Amphiregulin; AR; SDGF; AREG; AREGB; CRDGF; MGC13647
Accession	P15514
Species	Human
Amino Acid Range	Ser101-Lys187
Molecular Weight	The protein has a predicted MW of 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA.
	The purity of Human-AREG is greater than 95% as determined by SEC-HPLC.
	Human-AREG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.