Name: Human ARTN Protein (ARN-HM201)
Brand: KactusBio
SKU: ARN-HM201-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA.
Amino Acid Range: Ala108-Gly220

Catalog #ARN-HM201

Size

100UG

500UG

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Human ARTN Protein (ARN-HM201) Summary

Product Description	Recombinant Human ARTN Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ala108-Gly220.
Product Background	Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human ARTN Protein (ARN-HM201) Specifications/Details

Protein	ARTN
Synonyms	Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN
Accession	Q5T4W7-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala108-Gly220
Molecular Weight	The protein has a predicted MW of 39.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA.
Product Tag	N-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA.
	Human ARTN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human GFRA3, His Tag on Anti-His-Biosensor can bind Human ARTN, hFc Tag with an affinity constant of 5.95 nM as determined in BLI assay.
	The purity of Human ARTN is greater than 90% as determined by SEC-HPLC.

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