ELISA binding curve of human ARTN hFc-tag protein showing OD450 response to increasing concentrations of human GFRA3 His-tagged protein.
Human ARTN Protein (ARN-HM201)
Human ARTN Protein (ARN-HM201)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala108-Gly220
Catalog #ARN-HM201
Regular price $330.00
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Product Description

Recombinant Human ARTN Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala108-Gly220.

Product Background

Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

ARTN

Synonyms

Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala108-Gly220

Molecular Weight

The protein has a predicted MW of 39.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 4mM HCl (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test). Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test).
Human ARTN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ARTN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ARTN is greater than 95% as determined by SEC-HPLC. The purity of Human ARTN is greater than 95% as determined by SEC-HPLC.

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