Human ARTN Protein (ARN-HM201)
Human ARTN Protein (ARN-HM201)
Human ARTN Protein (ARN-HM201)
Human ARTN Protein (ARN-HM201)
Human ARTN Protein (ARN-HM201)
Human ARTN Protein (ARN-HM201)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala108-Gly220
Catalog #ARN-HM201
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Product Description
 Recombinant Human ARTN Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala108-Gly220.
Product Background
 Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.
Product Category
 Recombinant Protein
Target
 ARTN
Synonyms
 Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala108-Gly220
Molecular Weight
 The protein has a predicted MW of 39.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 N-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 4mM HCl (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl (pH2.8).
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test).
Human ARTN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ARTN is greater than 95% as determined by SEC-HPLC.
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