• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ile21-Met119
Catalog #B2M-HM101
Price
Regular price $21450
Regular price Sale price $21450
/
Size
Avantor VWR Fisher Scientific

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Human B2M/beta 2-Microglobulin Protein (B2M-HM101) Summary

Product Description

 Recombinant Human B2M/beta 2-Microglobulin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Met119.

Product Background

 The genetic and functional analysis of β2-microglobulin (B2M), a component of the HLA class-I complex.Acquired homozygous loss of B2M that caused lack of cell-surface HLA Class I expression in the tumor and a matched patient-derived xenograft (PDX). Downregulation of B2M was also found in two additional PDXs established from ICI-resistant tumors.

Human B2M/beta 2-Microglobulin Protein (B2M-HM101) Specifications/Details

Protein
 B2M/beta 2-Microglobulin
Synonyms
 Beta-2-microglobulin;B2M;β-2-microglobulin;b-2-microglobulin; b2-microglobulin
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ile21-Met119
Molecular Weight
 The protein has a predicted MW of 12.8 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human B2M on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human B2M on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B2M is greater than 95% as determined by SEC-HPLC. The purity of Human B2M is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...