Human B7-1/CD80 Protein (B71-HM280)
Human B7-1/CD80 Protein (B71-HM280)
Human B7-1/CD80 Protein (B71-HM280)
Human B7-1/CD80 Protein (B71-HM280)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). The affinity constant of 43.61 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val35-Asn242
Catalog #B71-HM280
Regular price $240.00
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Product Description

Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val35-Asn242.

Product Background

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

B7-1/CD80

Synonyms

CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Val35-Asn242

Molecular Weight

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). The affinity constant of 43.61 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 uM as determined in SPR assay (Biacore T200). Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 uM as determined in SPR assay (Biacore T200).
Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).
Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-1 is greater than 95% as determined by SEC-HPLC. The purity of Human B7-1 is greater than 95% as determined by SEC-HPLC.

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