Human B7-1/CD80 Protein (B71-HM280)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). The affinity constant of 43.61 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Val35-Asn242

Catalog: B71-HM280

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Product Description	Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val35-Asn242.
Product Background	Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Product Category	Recombinant Protein

Target	B7-1/CD80
Synonyms	CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
Gene/Protein ID	P33681-1
Species	Human
Exact Sequence	Val35-Asn242
Molecular Weight	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). The affinity constant of 43.61 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 uM as determined in SPR assay (Biacore T200).
	Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).
	The purity of Human B7-1 is greater than 95% as determined by SEC-HPLC.
	Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.