Human B7-1/CD80 Protein (B71-HM480)
Catalog: B71-HM480
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| Product Description | Recombinant Human B7-1/CD80 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Val35-Asn242. |
| Product Category | Recombinant Protein |
| Target | B7-1/CD80 |
| Synonyms | CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7 |
| Gene/Protein ID | P33681-1 |
| Species | Human |
| Exact Sequence | Val35-Asn242 |
| Molecular Weight | The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human B7-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 0.12μg/ml determined by ELISA. The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200). |
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Immobilized Human B7-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 0.12μg/ml determined by ELISA. |
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The purity of Human B7-1 is greater than 95% as determined by SEC-HPLC. |
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Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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