Human B7-1/CD80 Protein (B71-HM480)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test). The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val35-Asn242

Catalog: B71-HM480

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Product Description Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val35-Asn242.

Product Background

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.

Product Category

Recombinant Protein

Target

B7-1/CD80
Synonyms CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7

Gene/Protein ID

P33681-1

Species

Human

Exact Sequence

Val35-Asn242
Molecular Weight The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test). The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test).
The purity of- Human B7-1 is greater than 95% as determined by SEC-HPLC.
Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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