SPR sensorgram showing multiple binding curves at different analyte concentrations, illustrating real-time interaction kinetics between biomolecules.
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test). The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val35-Asn242
Catalog #B71-HM480
Regular price $247.50
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Product Description

Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val35-Asn242.

Product Background

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

B7-1/CD80

Synonyms

CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Val35-Asn242

Molecular Weight

The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test). The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200). Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test). Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test).
Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of- Human B7-1 is greater than 95% as determined by SEC-HPLC. The purity of- Human B7-1 is greater than 95% as determined by SEC-HPLC.

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