Human B7-1/CD80 Protein (B71-HM480)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human B7-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 0.12μg/ml determined by ELISA. The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val35-Asn242

Catalog: B71-HM480

$282.00
Size:

Order now get it on

Product Description Recombinant Human B7-1/CD80 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Val35-Asn242.
Product Category Recombinant Protein
Target B7-1/CD80
Synonyms CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
Gene/Protein ID P33681-1
Species Human
Exact Sequence Val35-Asn242
Molecular Weight The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human B7-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 0.12μg/ml determined by ELISA. The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
Immobilized Human B7-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 0.12μg/ml determined by ELISA.
The purity of  Human B7-1 is greater than 95% as determined by SEC-HPLC.
Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in