Human B7-1/CD80 Protein (B71-HM480)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test). The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val35-Asn242
Catalog #B71-HM480
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Product Description
 Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val35-Asn242.
Product Background
 Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Product Category
 Recombinant Protein
Target
 B7-1/CD80
Synonyms
 CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Val35-Asn242
Molecular Weight
 The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test). The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test).
Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of- Human B7-1 is greater than 95% as determined by SEC-HPLC.
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