Human B7-2/CD86 Protein (B72-HM286)
Catalog: B72-HM286
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Product Description | Recombinant Human B7-2/CD86 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Leu26-Pro247. |
Product Category | Recombinant Protein |
Target | B7-2/CD86 |
Synonyms | CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2 |
Gene/Protein ID | P42081-1 |
Species | Human |
Exact Sequence | Leu26-Pro247 |
Molecular Weight | The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human CTLA-4, His Tag at 5μg/ml (100μl/Well). Dose response curve for Human B7-2, hFc Tag with the EC50 of 0.36μg/ml determined by ELISA. See testing image for detail. |
Product Tag | C-hFc |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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