Product Description |
Recombinant Human B7-2/CD86 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Pro247. |
Product Background |
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
B7-2/CD86 |
Synonyms |
CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu26-Pro247 |
Molecular Weight |
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human B7-2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Human B7-2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. |
|
|
Human B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human B7-2 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed
