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Human B7-2/CD86 Protein, Ultra Low Endotoxin (B72-HM286-UL) Summary
Product Description |
Recombinant Human B7-2/CD86 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu26-Pro247. |
Product Background |
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human B7-2/CD86 Protein, Ultra Low Endotoxin (B72-HM286-UL) Specifications/Details
Protein |
B7-2/CD86 |
Synonyms |
CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu26-Pro247 |
Molecular Weight |
The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CTLA-4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CTLA-4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA. |
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Human B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human CTLA-4, His Tag on Anti-His-Biosensor can bind Human B7-2, hFc Tag with an affinity constant of 9.35 nM as determined in BLI assay . |
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The purity of Human B7-2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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