• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human B7-H2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2 ng/ml determined by ELISA (QC Test). The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Asp19-Ser258
Catalog #BH7-HM472
Price
Regular price $18150
Regular price Sale price $18150
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Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472) Summary

Product Description

 Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258.

Product Background

 B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.

Non-biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472) Specifications/Details

Protein
 B7-H2/ICOSLG
Synonyms
 CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50;B7H2B7-related protein 1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp19-Ser258
Molecular Weight
 The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human B7-H2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2 ng/ml determined by ELISA (QC Test). The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).
Immobilized Human B7-H2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2 ng/ml determined by ELISA (QC Test). Immobilized Human B7-H2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2 ng/ml determined by ELISA (QC Test).
Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H2 is greater than 90% as determined by SEC-HPLC. The purity of Human B7-H2 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
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