Human B7-H2/ICOSLG Protein (BH7-HM472)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA (QC Test). The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp19-Ser258

Catalog: BH7-HM472

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Product Description Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Asp19-Ser258.

Product Background

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.

Product Category

Recombinant Protein

Target

B7-H2/ICOSLG
Synonyms CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Accession

Species

Human

Amino Acid Range

Asp19-Ser258
Molecular Weight The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA (QC Test). The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1 EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).
Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA (QC Test).
The purity of Human B7-H2 is greater than 95% as determined by SEC-HPLC.
Human B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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