Human B7-H2/ICOSLG Protein (BH7-HM472)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-H2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA. The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Asp19-Ser258

Catalog: BH7-HM472

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Product Description	Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258.
Product Category	Recombinant Protein

Target	B7-H2/ICOSLG
Synonyms	CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Gene/Protein ID	O75144
Species	Human
Exact Sequence	Asp19-Ser258
Molecular Weight	The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human B7-H2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA. The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).
	Immobilized Human B7-H2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA.
	The purity of Human B7-H2 is greater than 95% as determined by SEC-HPLC.
	Human B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.