Product Description |
Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258. |
Product Background |
B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. |
Product Category |
Recombinant Protein |
Target |
B7-H2/ICOSLG |
Synonyms |
CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp19-Ser258 |
Molecular Weight |
The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human B7-H2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2 ng/ml determined by ELISA (QC Test). The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE; >95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human B7-H2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2 ng/ml determined by ELISA (QC Test). |
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Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human B7-H2 is greater than 95% as determined by SEC-HPLC. |
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