Name: Human B7-H3 (4Ig) /B7-H3b Protein, Ultra Low Endotoxin (BH7-HM23B-UL)
Brand: KactusBio
SKU: BH7-HM23B-UL-100UG
Price: 327.0 USD
Availability: InStock

Human B7-H3 (4Ig) /B7-H3b Protein, Ultra Low Endotoxin (BH7-HM23B-UL)

Immobilized Human B7-H3 (4Ig), hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 52.3ng/ml determined by ELISA.

Human B7-H3 (4Ig) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-H3 (4Ig), hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 52.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly27-Thr461

Catalog #BH7-HM23B-UL

Size

100UG

500UG

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Product Description	Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly27-Thr461.
Product Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	B7-H3 (4Ig)/B7-H3b
Synonyms	B7-H3; CD276; PSEC0249;-4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276
Accession	Q5ZPR3-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly27-Thr461
Molecular Weight	The protein has a predicted MW of 73.4 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human B7-H3 (4Ig), hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 52.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human B7-H3 (4Ig), hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 52.3ng/ml determined by ELISA.
	Human B7-H3 (4Ig) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC.