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Human B7-H3/CD276 Protein, Ultra Low Endotoxin (BH7-HM173-UL) Summary
Product Description |
Recombinant Human B7-H3/CD276 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu29-Pro245. |
Product Background |
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers. B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets |
Human B7-H3/CD276 Protein, Ultra Low Endotoxin (BH7-HM173-UL) Specifications/Details
Protein |
B7-H3/CD276 |
Synonyms |
B7H3; B7-H3; CD276; PSEC0249;-UNQ309; PRO352; B7 homolog 3; CD276 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu29-Pro245 |
Molecular Weight |
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human B7-H3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 8.5 ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human B7-H3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 8.5 ng/ml determined by ELISA. |
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Human B7-H3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-B7-H3 Ab., hFc Tag on ProA-Biosensor can bind Human B7-H3, His Tag with an affinity constant of 0.73 µM as determined in BLI assay . |
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The purity of Human B7-H3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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