Human B7-H4 Protein (BH7-HM274)
Human B7-H4 Protein (BH7-HM274)
Human B7-H4 Protein (BH7-HM274)
Human B7-H4 Protein (BH7-HM274)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human B7-H4, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe29-Ala258
Catalog #BH7-HM274
/
Size

Order now, ships by:

Product Description
 Recombinant Human B7-H4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Ala258.
Product Background
 B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status-.
Product Category
 Recombinant Protein
Target
 B7-H4
Synonyms
 B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe29-Ala258
Molecular Weight
 The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human B7-H4, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human B7-H4, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
Human B7-H4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC.
Recently viewed