Name: Human B7-H5/Gi24/VISTA Protein (BH7-HM275)
Brand: KactusBio
SKU: BH7-HM275-100UG
Price: 210.0 USD
Availability: InStock

Human B7-H5/Gi24/VISTA Protein (BH7-HM275)

Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human VSIG3, His Tag with an affinity constant of 14.44 uM as determined in SPR assay (Biacore T200).

Immobilized Human B7-H5, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA (QC Test).

Human B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human B7-H5 is greater than 95% as determined by SEC-HPLC

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-H5, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA (QC Test). The affinity constant of 14.44 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Phe33-Ala194

Catalog #BH7-HM275

Size

100UG

500UG

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Product Description	Recombinant Human B7-H5/Gi24/VISTA Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe33-Ala194.
Product Background	B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	B7-H5/Gi24/VISTA
Synonyms	B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1
Accession	Q9H7M9
Species	Human
Biotinylated	no
Amino Acid Range	Phe33-Ala194
Molecular Weight	The protein has a predicted MW of 44.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human B7-H5, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA (QC Test). The affinity constant of 14.44 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human VSIG3, His Tag with an affinity constant of 14.44 uM as determined in SPR assay (Biacore T200).
	Immobilized Human B7-H5, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA (QC Test).
	Human B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human B7-H5 is greater than 95% as determined by SEC-HPLC