Product Description |
Recombinant Human B7-H5/Gi24/VISTA Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe33-Ala194. |
Product Background |
B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. |
Product Category |
Recombinant Protein |
Target |
B7-H5/Gi24/VISTA |
Synonyms |
B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe33-Ala194 |
Molecular Weight |
The protein has a predicted MW of 44.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human B7-H5, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA (QC Test). The affinity constant of 14.44 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human VSIG3, His Tag with an affinity constant of 14.44 uM as determined in SPR assay (Biacore T200). |
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|
Immobilized Human B7-H5, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA (QC Test). |
|
|
Human B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human B7-H5 is greater than 95% as determined by SEC-HPLC |
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