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Human B7-H5/Gi24/VISTA Protein, Ultra Low Endotoxin (BH7-HM175-UL) Summary
Product Description |
Recombinant Human B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe33-Ala194. |
Product Background |
B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human B7-H5/Gi24/VISTA Protein, Ultra Low Endotoxin (BH7-HM175-UL) Specifications/Details
Protein |
B7-H5/Gi24/VISTA |
Synonyms |
B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe33-Ala194 |
Molecular Weight |
The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 32-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human B7-H5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human B7-H5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. |
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Human B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-B7-H5 Ab., hFc Tag on ProA-Biosensor can bind Human B7-H5, His Tag with an affinity constant of 5.22 nM as determined in BLI assay . |
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The purity of Human B7-H5 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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