Name: Human B7-H6/NCR3LG1 Protein (BH7-HM276)
Brand: KactusBio
SKU: BH7-HM276-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.292 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Asp25-Ser262

Catalog #BH7-HM276

Size

100UG

500UG

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Human B7-H6/NCR3LG1 Protein (BH7-HM276) Summary

Product Description	Recombinant Human B7-H6/NCR3LG1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Asp25-Ser262.
Product Background	B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human B7-H6/NCR3LG1 Protein (BH7-HM276) Specifications/Details

Protein	B7-H6/NCR3LG1
Synonyms	B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6
Accession	Q68D85-1
Species	Human
Biotinylated	no
Amino Acid Range	Asp25-Ser262
Molecular Weight	The protein has a predicted MW of 53.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.292 uM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 uM as determined in SPR assay (Biacore T200).
	Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).
	Human B7-H6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human B7-H6, hFc Tag on ProA-Biosensor, can bind Human NKp30, His Tag with an affinity constant of 0.51 µM as determined in BLI assay (Gator).
	The purity of Human B7-H6 is greater than 95% as determined by SEC-HPLC.

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