Human B7-H6/NCR3LG1 Protein (BH7-HM276)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.292 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp25-Ser262
Catalog #BH7-HM276
Regular price $270.00
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Product Description
Recombinant Human B7-H6/NCR3LG1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp25-Ser262.
Product Background
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
Product Category
Recombinant Protein
Target
B7-H6/NCR3LG1
Synonyms
B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Asp25-Ser262
Molecular Weight
The protein has a predicted MW of 53.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.292 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 uM as determined in SPR assay (Biacore T200).
Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).
Human B7-H6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H6 is greater than 95% as determined by SEC-HPLC.

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