Human B7-H7/HHLA2 Protein (BH7-HM177) Summary
Product Description |
Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Asn344. |
Product Background |
B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human B7-H7/HHLA2 Protein (BH7-HM177) Specifications/Details
Protein |
B7-H7/HHLA2 |
Synonyms |
B7H7; B7-H7; HHLA2; B7 Homolog 7 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile23-Asn344 |
Molecular Weight |
The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human B7-H7, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
Other Available Proteins:
Finding available proteins...
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