Human B7-H7/HHLA2 Protein (BH7-HM177)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ile23-Asn344

Catalog: BH7-HM177

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Product Description Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Asn344.

Product Background

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Product Category

Recombinant Protein

Target

B7-H7/HHLA2
Synonyms B7H7; B7-H7; HHLA2; B7 Homolog 7
Accession

Species

Human

Amino Acid Range

Ile23-Asn344
Molecular Weight The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test).
The purity of Human B7-H7 is greater than 95% as determined by SEC-HPLC.
Human B7-H7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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