Human B7-H7/HHLA2 Protein (BH7-HM177)
Human B7-H7/HHLA2 Protein (BH7-HM177)
Human B7-H7/HHLA2 Protein (BH7-HM177)
Human B7-H7/HHLA2 Protein (BH7-HM177)
Human B7-H7/HHLA2 Protein (BH7-HM177)
Human B7-H7/HHLA2 Protein (BH7-HM177)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human B7-H7, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ile23-Asn344
Catalog #BH7-HM177
Regular price $297.00
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Product Description

Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Asn344.

Product Background

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

B7-H7/HHLA2

Synonyms

B7H7; B7-H7; HHLA2; B7 Homolog 7

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ile23-Asn344

Molecular Weight

The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human B7-H7, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human B7-H7, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA (QC Test). Immobilized Human B7-H7, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA (QC Test).
Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H7 is greater than 95% as determined by SEC-HPLC. The purity of Human B7-H7 is greater than 95% as determined by SEC-HPLC.

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