ELISA binding curve for human B7-H7, His tag, showing a sigmoidal dose-response with human CD28H, hFc tag. Mean absorbance (OD450) is plotted against the log concentration of human CD28H, hFc tag (µg/ml).
Human B7-H7/HHLA2 Protein (BH7-HM177)
Human B7-H7/HHLA2 Protein (BH7-HM177)
Human B7-H7/HHLA2 Protein (BH7-HM177)
Human B7-H7/HHLA2 Protein (BH7-HM177)
Human B7-H7/HHLA2 Protein (BH7-HM177)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human B7-H7, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ile23-Asn344
Catalog #BH7-HM177
Regular price $297.00
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Human B7-H7/HHLA2 Protein (BH7-HM177) Summary

Product Description

Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Asn344.

Product Background

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human B7-H7/HHLA2 Protein (BH7-HM177) Specifications/Details

Protein
B7-H7/HHLA2
Synonyms
B7H7; B7-H7; HHLA2; B7 Homolog 7
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ile23-Asn344
Molecular Weight
The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human B7-H7, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA (QC Test).
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human B7-H7, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA (QC Test). Immobilized Human B7-H7, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA (QC Test).
Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H7 is greater than 90% as determined by SEC-HPLC. The purity of Human B7-H7 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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