Human B7-H7/HHLA2 Protein (BH7-HM277)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test). The affinity constant of 334 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ile23-Asn344
Catalog #BH7-HM277
Regular price $270.00
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Product Description

Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile23-Asn344.

Product Background

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Product Category

Recombinant Protein

Protein

B7-H7/HHLA2

Synonyms

B7H7; B7-H7; HHLA2; B7 Homolog 7

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ile23-Asn344

Molecular Weight

The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test). The affinity constant of 334 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200). Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).
Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test). Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test).
Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H7 is greater than 95% as determined by SEC-HPLC. The purity of Human B7-H7 is greater than 95% as determined by SEC-HPLC.

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