Name: Human B7-H7/HHLA2 Protein (BH7-HM277)
Brand: KactusBio
SKU: BH7-HM277-100UG
Price: 270.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test). The affinity constant of 334 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ile23-Asn344

Catalog #BH7-HM277

Size

100UG

500UG

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Product Description	Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile23-Asn344.
Product Background	B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Product Category	Recombinant Protein

Protein	B7-H7/HHLA2
Synonyms	B7H7; B7-H7; HHLA2; B7 Homolog 7
Accession	Q9UM44-1
Species	Human
Biotinylated	no
Amino Acid Range	Ile23-Asn344
Molecular Weight	The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test). The affinity constant of 334 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).
	Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test).
	Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human B7-H7 is greater than 95% as determined by SEC-HPLC.

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