• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test). The affinity constant of 334 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ile23-Asn344
Catalog #BH7-HM277
Price
Regular price $29700
Regular price Sale price $29700
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Size
Avantor VWR Fisher Scientific

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Human B7-H7/HHLA2 Protein (BH7-HM277) Summary

Product Description

 Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile23-Asn344.

Product Background

 B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Human B7-H7/HHLA2 Protein (BH7-HM277) Specifications/Details

Protein
 B7-H7/HHLA2
Synonyms
 B7H7; B7-H7; HHLA2; B7 Homolog 7
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ile23-Asn344
Molecular Weight
 The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test). The affinity constant of 334 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200). Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).
Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test). Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test).
Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H7 is greater than 90% as determined by SEC-HPLC. The purity of Human B7-H7 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...