Human BACE-1 Protein (BAE-HM101)
Catalog: BAE-HM101
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| Product Description | Recombinant Human BACE-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr22-Thr457. |
Product Background |
The beta-site amyloid precursor protein cleaving enzyme-1 (BACE-1) initiates the generation of amyloid-β (Aβ), and the amyloid cascade leading to amyloid plaque deposition, neurodegeneration, and dementia in Alzheimer's disease (AD). Clinical failures of anti-Aβ therapies in dementia stages suggest that treatment has to start in the early, asymptomatic disease states. |
Product Category |
Recombinant Protein |
Target |
BACE-1 |
| Synonyms | Beta-secretase 1; ASP2; Asp 2; BACE1; BACE; FLJ90568; HSPC104; KIAA1149; Memapsin-2; BACE-1; EC 3.4.23; EC 3.4.23.46 |
Gene/Protein ID |
NP_036236.1 |
Species |
Human |
Exact Sequence |
Thr22-Thr457 |
| Molecular Weight | The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >3.5 pmol/min/µg. |
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The purity of Human BACE-1 is greater than 95% as determined by SEC-HPLC. |
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Human BACE-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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