Human BAFF/TNFSF13B/CD257 Protein (BAF-HM214)
Catalog: BAF-HM214
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Product Description | Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala134-Leu285. |
Product Category | Recombinant Protein |
Target | BAFF/TNFSF13B/CD257 |
Synonyms | BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1 |
Gene/Protein ID | Q9Y275-1 |
Species | Human |
Exact Sequence | Ala134-Leu285 |
Molecular Weight | The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human BCMA Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | N-monomeric hFc |
Express System | HEK293 |
Purity | > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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