Human BAFF/TNFSF13B/CD257 Protein (BAF-HM214)
  • Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala134-Leu285
Catalog #BAF-HM214
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Product Description
 Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala134-Leu285.
Product Background
 B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Product Category
 Recombinant Protein
Target
 BAFF/TNFSF13B/CD257
Synonyms
 BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala134-Leu285
Molecular Weight
 The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-monomeric hFc
Express System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Immobilized Human BAFF, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.7ng/ml determined by ELISA.
Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BAFF is greater than 95% as determined by SEC-HPLC.

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