Human BAFF/TNFSF13B/CD257 Protein (BAF-HM214)

  • Express System: Expi293
  • Product Tag: N-monomeric hFc
  • Purity: > 90% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
  • Activity: Immobilized Human BCMA Trimer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 17ng/ml determined by ELISA. The affinity constant of 5.83 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala134-Leu285

Catalog: BAF-HM214

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Product Description Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from Expi293 with hFc tag at the N-terminal.; It contains Ala134-Leu285 .
Product Category Recombinant Protein
Target BAFF/TNFSF13B/CD257
Synonyms BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Gene/Protein ID Q9Y275-1
Species Human
Exact Sequence Ala134-Leu285
Molecular Weight The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human BCMA Trimer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 17ng/ml determined by ELISA. The affinity constant of 5.83 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-monomeric hFc
Express System Expi293
Purity > 90% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 μM as determined in SPR assay (Biacore T200).
Immobilized Human BCMA Trimer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 17ng/ml determined by ELISA.
The purity of Human BAFF is greater than 95% as determined by SEC-HPLC.
Human BAFF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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