Name: Human BAFF/TNFSF13B/CD257 Protein (BAF-HM214)
Brand: KactusBio
SKU: BAF-HM214-100UG
Price: 346.5 USD
Availability: InStock

Human BAFF/TNFSF13B/CD257 Protein (BAF-HM214)

Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).

Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).

Immobilized Human BAFF, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.7ng/ml determined by ELISA.

Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human BAFF is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-monomeric hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala134-Leu285

Catalog #BAF-HM214

Size

100UG

500UG

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Product Description	Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with monomeric Fc tag at the N-Terminus.It contains Ala134-Leu285.
Product Background	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	BAFF/TNFSF13B/CD257
Synonyms	BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession	Q9Y275-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala134-Leu285
Molecular Weight	The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-monomeric hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
	Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
	Immobilized Human BAFF, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.7ng/ml determined by ELISA.
	Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human BAFF is greater than 95% as determined by SEC-HPLC.