Human BAFF/TNFSF13B/CD257 Protein (BAF-HM214)

Express System: HEK293
Product Tag: N-monomeric hFc
Purity: > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: Immobilized Human BCMA Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala134-Leu285

Catalog: BAF-HM214

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Product Description	Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala134-Leu285.
Product Background	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Product Category	Recombinant Protein

Target	BAFF/TNFSF13B/CD257
Synonyms	BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Gene/Protein ID	Q9Y275-1
Species	Human
Exact Sequence	Ala134-Leu285
Molecular Weight	The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human BCMA Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-monomeric hFc
Express System	HEK293
Purity	> 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
	Immobilized Human BCMA Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
	The purity of Human BAFF is greater than 95% as determined by SEC-HPLC.
	Human BAFF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.