Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM112)
Catalog: BAF-HM112
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| Product Description | Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.; It contains Thr141-Leu285. |
| Product Category | Recombinant Protein |
| Target | BAFF/TNFSF13B/CD257 Trimer |
| Synonyms | BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1 |
| Gene/Protein ID | Q9Y275 |
| Species | Human |
| Exact Sequence | Thr141-Leu285 |
| Molecular Weight | The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human BAFF Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA. The affinity constant of 2.68 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-His-Flag |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human BAFF Trimer, His-Flag Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 2.68 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human BAFF Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA. |
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Immobilized Human BAFF Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. |
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The purity of Human BAFF Trimer is greater than 95% as determined by SEC-HPLC. |
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Human BAFF Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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