Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM112)
Catalog: BAF-HM112
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Product Description | Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.; It contains Thr141-Leu285. |
Product Category | Recombinant Protein |
Target | BAFF/TNFSF13B/CD257 Trimer |
Synonyms | BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1 |
Gene/Protein ID | Q9Y275 |
Species | Human |
Exact Sequence | Thr141-Leu285 |
Molecular Weight | The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human BAFF Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA. The affinity constant of 2.68 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | N-His-Flag |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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