Name: Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM112)
Brand: KactusBio
SKU: BAF-HM112-100UG
Price: 330.0 USD
Availability: InStock

Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM112)

Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200).

Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test).

Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.

Human BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human BAFF Trimer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test). The affinity constant of 6.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Thr141-Leu285

Catalog #BAF-HM112

Size

100UG

500UG

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Product Description	Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Thr141-Leu285.
Product Background	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Product Category	Recombinant Protein / Antibody discovery

Protein	BAFF/TNFSF13B/CD257 Trimer
Synonyms	BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession	Q9Y275-1
Species	Human
Biotinylated	no
Amino Acid Range	Thr141-Leu285
Molecular Weight	The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test). The affinity constant of 6.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200).
	Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test).
	Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
	Human BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human BAFF Trimer is greater than 95% as determined by SEC-HPLC.