Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM112)

  • Express System: Expi293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human BAFF Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA. The affinity constant of 2.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr141-Leu285

Catalog: BAF-HM112

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Product Description Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.; It contains Thr141-Leu285.
Product Category Recombinant Protein
Target BAFF/TNFSF13B/CD257 Trimer
Synonyms BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Gene/Protein ID Q9Y275
Species Human
Exact Sequence Thr141-Leu285
Molecular Weight The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human BAFF Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA. The affinity constant of 2.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFF Trimer, His-Flag Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 2.68 nM as determined in SPR assay (Biacore T200).
Immobilized Human BAFF Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA.
Immobilized Human BAFF Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
The purity of Human BAFF Trimer is greater than 95% as determined by SEC-HPLC.
Human BAFF Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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