Name: Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM213)
Brand: KactusBio
SKU: BAF-HM213-100UG
Price: 363.0 USD
Availability: InStock

Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM213)

Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).

Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).

Human BAFF (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human BAFF (Trimer) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-monomeric hFc-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Thr141-Leu285

Catalog #BAF-HM213

Size

100UG

500UG

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Product Description	Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with monomeric Fc tag and Flag tag at the N-Terminus.It contains Thr141-Leu285 trimer design.
Product Background	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	BAFF/TNFSF13B/CD257
Synonyms	BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession	Q9Y275-1
Species	Human
Biotinylated	no
Amino Acid Range	Thr141-Leu285
Molecular Weight	The protein has a predicted MW of 74.9 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-monomeric hFc-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
	Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
	Human BAFF (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human BAFF (Trimer) is greater than 95% as determined by SEC-HPLC.