Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM213)
  • Express System: HEK293
  • Product Tag: N-monomeric hFc-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr141-Leu285
Catalog #BAF-HM213
Regular price $363.00
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Product Description

Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with monomeric Fc tag and Flag tag at the N-Terminus.It contains Thr141-Leu285 trimer design.

Product Background

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

BAFF/TNFSF13B/CD257

Synonyms

BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Thr141-Leu285

Molecular Weight

The protein has a predicted MW of 74.9 kDa same as Bis-Tris PAGE result.

Activity

Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-monomeric hFc-Flag

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
Human BAFF (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BAFF (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BAFF (Trimer) is greater than 95% as determined by SEC-HPLC. The purity of Human BAFF (Trimer) is greater than 95% as determined by SEC-HPLC.

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