Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM213)

  • Express System: Expi293
  • Product Tag: N-monomeric hFc,N-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 94% as determined by HPLC
  • Activity: Immobilized Human BAFF (Trimer) , hFc Tag at 5_g/ml (100_l/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3_g/ml determined by ELISA. The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr141-Leu285

Catalog: BAF-HM213

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Product Description Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from Expi293 with monomeric hFc tag and Flag tag at the N-terminal.It contains Thr141-Leu285.
Product Category Recombinant Protein
Target BAFF/TNFSF13B/CD257 Trimer
Synonyms BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Gene/Protein ID Q9Y275-1
Species Human
Exact Sequence Thr141-Leu285
Molecular Weight The protein has a predicted MW of 74.9 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human BAFF (Trimer) , hFc Tag at 5_g/ml (100_l/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3_g/ml determined by ELISA. The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-monomeric hFc,N-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 94% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Immobilized Human BAFF (Trimer) , hFc Tag at 5_g/ml (100_l/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3_g/ml determined by ELISA.
The purity of Human BAFF (Trimer) is greater than 94% as determined by SEC-HPLC.
Human BAFF (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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