Product Description |
Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with monomeric hFc tag and Flag tag at the N-Terminus.It contains Thr141-Leu285. |
Product Background |
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. |
Product Category |
Recombinant Protein |
Target |
BAFF/TNFSF13B/CD257 Trimer |
Synonyms |
BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr141-Leu285 |
Molecular Weight |
The protein has a predicted MW of 74.9 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-monomeric hFc-Flag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). |
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Human BAFF (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human BAFF (Trimer) is greater than 90% as determined by SEC-HPLC. |
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