Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM213)

  • Express System: Expi293
  • Product Tag: N-monomeric hFc,N-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 94% as determined by HPLC
  • Activity: Immobilized Human BAFF (Trimer) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3μg/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr141-Leu285

Catalog: BAF-HM213

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Product Description Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from Expi293 with monomeric hFc tag and Flag tag at the N-terminal.It contains Thr141-Leu285.
Product Category Recombinant Protein
Target BAFF/TNFSF13B/CD257 Trimer
Synonyms BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Gene/Protein ID Q9Y275-1
Species Human
Exact Sequence Thr141-Leu285
Molecular Weight The protein has a predicted MW of 74.9 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human BAFF (Trimer) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3μg/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-monomeric hFc,N-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 94% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Immobilized Human BAFF (Trimer) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3μg/ml determined by ELISA (QC Test).
The purity of Human BAFF (Trimer) is greater than 94% as determined by SEC-HPLC.
Human BAFF (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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