Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM213)

  • Express System: HEK293
  • Product Tag: N-monomeric hFc-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 94% as determined by HPLC
  • Activity: Immobilized Human BAFF (Trimer) , hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.45ug/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr141-Leu285

Catalog: BAF-HM213

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Product Description Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with monomeric hFc tag and Flag tag at the N-Terminus.It contains Thr141-Leu285.

Product Background

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Product Category

Recombinant Protein

Target

BAFF/TNFSF13B/CD257 Trimer
Synonyms BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession

Species

Human

Amino Acid Range

Thr141-Leu285
Molecular Weight The protein has a predicted MW of 74.9 kDa same as Tris-Bis PAGE result.

Activity

Immobilized Human BAFF (Trimer) , hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.45ug/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-monomeric hFc-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 94% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Immobilized Human BAFF (Trimer) , hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.45ug/ml determined by ELISA (QC Test).
The purity of Human BAFF (Trimer) is greater than 94% as determined by SEC-HPLC.
Human BAFF (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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