Name: Human BAFFR/TNFRSF13C Protein (BAF-HM10R)
Brand: KactusBio
SKU: BAF-HM10R-100UG
Price: 379.5 USD
Availability: InStock

Human BAFFR/TNFRSF13C Protein (BAF-HM10R)

Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF Trimer, hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).

Immobilized Human BAFFR, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test).

Immobilized Human BAFFR, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA.

Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 90%.

Loaded Human BAFFR, His Tag on Anti-His-Biosensor can bind Human BAFF (Trimer), monomeric Fc-Flag Tag with an affinity constant of 0.98 nM as determined in BLI assay (Gator® Prime).

Express System: HEK293
Product Tag: C-His
Purity: > 90% as determined by Bis-Tris PAGE
Activity: Immobilized Human BAFFR, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ser7-Ala71

Catalog #BAF-HM10R

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human BAFFR/TNFRSF13C Protein (BAF-HM10R) Summary

Product Description	Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser7-Ala71.
Product Background	BAFFR is an atypical representative of the TNF-receptor super-family. BAFFR [BAFFR = B cell activating f actor of the TNF-f amily receptor; a.k.a. BR3, Bcmd] expression starts when the immature B cells develop to transitional B cells, which then receive BAFFR-dependent pro-survival signals to rescue them from premature cell death. At protein level, the BAFFR is expressed on the surface of all human peripheral B cell subsets except for plasma cells and for centroblasts located in the dark zone of germinal centers. Its expression is upregulated after the expression of functional B cell antigen receptors (BCR) in response to tonic BCR signaling, which enhances BAFFR expression by immature and transitional B cells
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human BAFFR/TNFRSF13C Protein (BAF-HM10R) Specifications/Details

Protein	BAFFR/TNFRSF13C
Synonyms	CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
Accession	Q96RJ3-1
Species	Human
Biotinylated	no
Amino Acid Range	Ser7-Ala71
Molecular Weight	The protein has a predicted MW of 7.6 kDa. Due to glycosylation, the protein migrates to 13-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BAFFR, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF Trimer, hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
	Immobilized Human BAFFR, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test).
	Immobilized Human BAFFR, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA.
	Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 90%.
	Loaded Human BAFFR, His Tag on Anti-His-Biosensor can bind Human BAFF (Trimer), monomeric Fc-Flag Tag with an affinity constant of 0.98 nM as determined in BLI assay (Gator® Prime).