Human BAFFR/TNFRSF13C Protein (BAF-HM10R)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human BAFFR, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA. The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser7-Ala71
Catalog #BAF-HM10R
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Product Description
 Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser7-Ala71.
Product Background
 BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF.
Product Category
 Recombinant Protein
Target
 BAFFR/TNFRSF13C
Synonyms
 CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser7-Ala71
Molecular Weight
 The protein has a predicted MW of 7.6 kDa. Due to glycosylation, the protein migrates to 13-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human BAFFR, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA. The affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF Trimer, hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Immobilized Human BAFFR, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA.
Immobilized Human BAFFR, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test).
Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

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