ELISA binding curve showing the dose-dependent interaction between immobilized Human BAFF Trimer, His Tag, and Human BAFFR, hFc Tag. The plot presents mean absorbance (OD450) versus the log concentration of Human BAFFR (µg/ml), displaying a sigmoidal response.
Human BAFFR/TNFRSF13C Protein (BAF-HM201)
Human BAFFR/TNFRSF13C Protein (BAF-HM201)
Human BAFFR/TNFRSF13C Protein (BAF-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.
  • Amino Acid Range: Ser7-Ala71
Catalog #BAF-HM201
Regular price $346.50
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Human BAFFR/TNFRSF13C Protein (BAF-HM201) Summary

Product Description

Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser7-Ala71.

Product Background

BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human BAFFR/TNFRSF13C Protein (BAF-HM201) Specifications/Details

Protein
BAFFR/TNFRSF13C
Synonyms
CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser7-Ala71
Molecular Weight
The protein has a predicted MW of 33.3 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.
Product Tag
C-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA. Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.
Human BAFFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BAFFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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