Regular price
$330.00
Order by 4pm ET for delivery on:
Human BAFFR/TNFRSF13C Protein (BAF-HM60R) Summary
Product Description |
Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with Llama IgG2b Fc tag at the C-terminus.It contains Ser7-Ala71. |
Product Background |
BAFFR is an atypical representative of the TNF-receptor super-family. BAFFR [BAFFR = B cell activating f actor of the TNF-f amily receptor; a.k.a. BR3, Bcmd] expression starts when the immature B cells develop to transitional B cells, which then receive BAFFR-dependent pro-survival signals to rescue them from premature cell death. At protein level, the BAFFR is expressed on the surface of all human peripheral B cell subsets except for plasma cells and for centroblasts located in the dark zone of germinal centers. Its expression is upregulated after the expression of functional B cell antigen receptors (BCR) in response to tonic BCR signaling, which enhances BAFFR expression by immature and transitional B cells |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human BAFFR/TNFRSF13C Protein (BAF-HM60R) Specifications/Details
Protein |
BAFFR/TNFRSF13C |
Synonyms |
CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser7-Ala71 |
Molecular Weight |
The protein has a predicted MW of 33.85 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human BAFFR, Llama Fc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His-Avi Tag (Cat. BAF-HM4AFB) with the EC50 of 2.5ng/ml determined by ELISA. |
Product Tag |
C-Llama IgG2b Fc |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human BAFFR, Llama Fc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His-Avi Tag (Cat. BAF-HM4AFB) with the EC50 of 2.5ng/ml determined by ELISA. |
|
|
Immobilized Human BAFFR, Llama Fc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. |
|
|
Human BAFFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
