Human BCAM Protein (BCA-HM10M)
Catalog: BCA-HM10M
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| Product Description | Recombinant Human BCAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu32-Ala547. |
Product Background |
Lutheran/basal cell adhesion molecule (Lu/BCAM) is a transmembrane adhesion molecule expressed by erythrocytes and endothelial cells that can interact with the extracellular matrix protein laminin-α5. In sickle cell disease, Lu/BCAM is thought to contribute to adhesion of sickle erythrocytes to the vascular wall, especially during vaso-occlusive crises. |
Product Category |
Recombinant Protein |
Target |
BCAM |
Gene/Protein ID |
P50895 |
Species |
Human |
Exact Sequence |
Glu32-Ala547 |
| Molecular Weight | The protein has a predicted MW of 57.32 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Human BCAM is greater than 95% as determined by SEC-HPLC. |
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Human BCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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