Name: Human BDCA-2 Protein (BCA-HM102)
Brand: KactusBio
SKU: BCA-HM102-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Phe46-Ile213

Catalog #BCA-HM102

Size

100UG

500UG

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Product Description	Recombinant Human BDCA-2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Phe46-Ile213.
Product Background	BDCA-2 is a novel type II C-type lectin specifically expressed by plasmacytoid dendritic cells (PDCs) that can internalize antigen for presentation to T cells. Furthermore, signaling via BDCA-2 may play a role in switching from interferon (IFN)-α/β-controlled to interleukin (IL)-12-controlled immune response pathways, as triggering of BDCA-2 potently inhibits secretion of IFN-α/β by PDCs and thereby promotes IL-12 p70 production in PDCs and other cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	BDCA-2
Synonyms	BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303;
Accession	Q8WTT0-1
Species	Human
Biotinylated	no
Amino Acid Range	Phe46-Ile213
Molecular Weight	The protein has a predicted MW of 20.93 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 20mM Tris, 500mM NaCl, 200mM L-arginine (pH 8.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-BDCA-2 Antibody captured on CM5 Chip via Protein A can bind Human BDCA-2, His Tag with an affinity constant of 2.97 nM as determined in SPR assay (Biacore T200) (Routine Test).
	Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test).
	Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.