Human BDCA-2 Protein (BCA-HM102)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Phe46-Ile213
Catalog #BCA-HM102
Regular price $315.00
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Product Description

Recombinant Human BDCA-2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Phe46-Ile213.

Product Background

BDCA-2 is a novel type II C-type lectin specifically expressed by plasmacytoid dendritic cells (PDCs) that can internalize antigen for presentation to T cells. Furthermore, signaling via BDCA-2 may play a role in switching from interferon (IFN)-α/β-controlled to interleukin (IL)-12-controlled immune response pathways, as triggering of BDCA-2 potently inhibits secretion of IFN-α/β by PDCs and thereby promotes IL-12 p70 production in PDCs and other cells.

Product Category

Recombinant Protein / Antibody discovery

Protein

BDCA-2

Synonyms

BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303;

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Phe46-Ile213

Molecular Weight

The protein has a predicted MW of 20.93 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in 20mM Tris, 500mM NaCl, 200mM L-arginine (pH 8.5).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Anti-BDCA-2 Antibody captured on CM5 Chip via Protein A can bind Human BDCA-2, His Tag with an affinity constant of 2.97 nM as determined in SPR assay (Biacore T200) (Routine Test). Anti-BDCA-2 Antibody captured on CM5 Chip via Protein A can bind Human BDCA-2, His Tag with an affinity constant of 2.97 nM as determined in SPR assay (Biacore T200) (Routine Test).
Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test).
Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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