• Express System: HEK293
  • Product Tag: N-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human BDCA-2, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
  • Amino Acid Range: Phe46-Ile213
Catalog #BCA-HM302
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human BDCA-2 Protein (BCA-HM302) Summary

Product Description

 Recombinant Human BDCA-2 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Phe46-Ile213.

Product Background

 BDCA-2 is a novel type II C-type lectin specifically expressed by plasmacytoid dendritic cells (PDCs) that can internalize antigen for presentation to T cells. Furthermore, signaling via BDCA-2 may play a role in switching from interferon (IFN)-α/β-controlled to interleukin (IL)-12-controlled immune response pathways, as triggering of BDCA-2 potently inhibits secretion of IFN-α/β by PDCs and thereby promotes IL-12 p70 production in PDCs and other cells.

Human BDCA-2 Protein (BCA-HM302) Specifications/Details

Protein
 BDCA-2
Synonyms
 BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe46-Ile213
Molecular Weight
 The protein has a predicted MW of 46.39 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human BDCA-2, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
Product Tag
 N-mFc
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl, 200mM L-arginine (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human BDCA-2, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. Immobilized Human BDCA-2, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
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