Human Beta Klotho Protein (KLB-HM101)

SKU: KLB-HM101-100UG

Human Beta Klotho Protein (KLB-HM101)

SKU: KLB-HM101-100UG
Human Beta Klotho Protein (KLB-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Met30-Thr983
Catalog #KLB-HM101
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Size
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Human Beta Klotho Protein (KLB-HM101) Summary

Product Description

 Recombinant Human Beta Klotho Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met30-Thr983.

Product Background

 Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Beta Klotho Protein (KLB-HM101) Specifications/Details

Protein
 Beta Klotho
Synonyms
 betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; MGC142213;βKlotho; β-klotho; klotho β like; Klotho β
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met30-Thr983
Molecular Weight
 The protein has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200). Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test). Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test).
Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC50 of 34.6ng/ml determined by ELISA (QC Test). Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC50 of 34.6ng/ml determined by ELISA (QC Test).
Immobilized Human FGF21, mFc Tag (Cat. FGF-HM621) at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75ug/ml determined by ELISA. Immobilized Human FGF21, mFc Tag (Cat. FGF-HM621) at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75ug/ml determined by ELISA.
Human Beta Klotho on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human Beta Klotho on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Loaded Anti-Beta Klotho Antibody1, hFc Tag on ProA-Biosensor can bind Human Beta Klotho, His Tag with an affinity constant of 9.40 nM as determined in BLI assay . Loaded Anti-Beta Klotho Antibody1, hFc Tag on ProA-Biosensor can bind Human Beta Klotho, His Tag with an affinity constant of 9.40 nM as determined in BLI assay .
Loaded Anti-Beta Klotho Antibody2, hFc Tag on ProA-Biosensor can bind Human Beta Klotho, His Tag with an affinity constant of 0.40 nM as determined in BLI assay . Loaded Anti-Beta Klotho Antibody2, hFc Tag on ProA-Biosensor can bind Human Beta Klotho, His Tag with an affinity constant of 0.40 nM as determined in BLI assay .
Loaded Human Beta Klotho, His Tag on Aint-His-Biosensor can bind Human FGF21, mFc Tag (Cat. FGF-HM621) with an affinity constant of 0.16 nM as determined in BLI assay . Loaded Human Beta Klotho, His Tag on Aint-His-Biosensor can bind Human FGF21, mFc Tag (Cat. FGF-HM621) with an affinity constant of 0.16 nM as determined in BLI assay .
The purity of Human Beta Klotho is greater than 95% as determined by SEC-HPLC. The purity of Human Beta Klotho is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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