Human BST1 Protein (BST-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BST1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly29-Ala293

Catalog: BST-HM101

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly29-Ala293.
Product Background	BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.
Product Category	Recombinant Protein

Target	BST1
Synonyms	Antigen BP3; BST-1; Bp-3; Bp3; Ly65; Bst1; BST1; CD157
Gene/Protein ID	Q10588-1
Species	Human
Exact Sequence	Gly29-Ala293
Molecular Weight	The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human BST1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human BST1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA.
	Human BST1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.