Product Description |
Recombinant Human BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly29-Ala293. |
Product Background |
BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
BST1 |
Synonyms |
Antigen BP3; BST-1; Bp-3; Bp3; Ly65; Bst1; BST1; CD157 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly29-Ala293 |
Molecular Weight |
The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human BST1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Human BST1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. |
|
|
Human BST1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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