Name: Human BST1 Protein (BST-HM101)
Brand: KactusBio
SKU: BST-HM101-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human BST1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
Amino Acid Range: Gly29-Ala293

Catalog #BST-HM101

Size

100UG

500UG

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Human BST1 Protein (BST-HM101) Summary

Product Description	Recombinant Human BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly29-Ala293.
Product Background	BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human BST1 Protein (BST-HM101) Specifications/Details

Protein	BST1
Synonyms	Antigen BP3; BST-1; Bp-3; Bp3; Ly65; Bst1; BST1; CD157
Accession	Q10588-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly29-Ala293
Molecular Weight	The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BST1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human BST1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
	Human BST1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-BST1 Antibody, hFc Tag on ProA-Biosensor can bind Human BST1, His Tag with an affinity constant of 3.26 nM as determined in BLI assay .

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