Human BST2 Protein (BST-HM102)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human BST2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Asn49-Ser161

Catalog: BST-HM102

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Product Description	Recombinant Human BST2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asn49-Ser161.
Product Background	Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor.
Product Category	Recombinant Protein

Target	BST2
Synonyms	BST-2; HM1.24 antigen; CD317; BST2; NPC-A-7; PDCA-1; TETHERIN
Gene/Protein ID	Q10589-1
Species	Human
Exact Sequence	Asn49-Ser161
Molecular Weight	The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human BST2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human BST2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
	Human BST2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.