• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human BST2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Asn49-Ser161
Catalog #BST-HM102
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Human BST2 Protein (BST-HM102) Summary

Product Description

 Recombinant Human BST2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asn49-Ser161.

Product Background

 Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor.

Human BST2 Protein (BST-HM102) Specifications/Details

Protein
 BST2
Synonyms
 BST-2; HM1.24 antigen; CD317; BST2; NPC-A-7; PDCA-1; TETHERIN;NPC-A7; PDCA1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asn49-Ser161
Molecular Weight
 The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human BST2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human BST2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). Immobilized Human BST2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Human BST2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BST2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-BST2 Antibody, hFc Tag on ProA-Biosensor can bind Human BST2, His Tag with an affinity constant of 15.60 nM as determined in BLI assay. Loaded Anti-BST2 Antibody, hFc Tag on ProA-Biosensor can bind Human BST2, His Tag with an affinity constant of 15.60 nM as determined in BLI assay.
The purity of Human BST2 is greater than 90% as determined by SEC-HPLC. The purity of Human BST2 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...