Human BST2 Protein (BST-HM202)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BST2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BST2 Antibody, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA. determined by ELISA. See testing image for detail.
Exact Sequence: Asn49-Ser161

Catalog: BST-HM202

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Product Description	Recombinant Human BST2 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asn49-Ser161.
Product Background	Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor.
Product Category	Recombinant Protein

Target	BST2
Synonyms	BST-2; HM1.24 antigen; CD317; BST2; NPC-A-7; PDCA-1; TETHERIN
Gene/Protein ID	Q10589-1
Species	Human
Exact Sequence	Asn49-Ser161
Molecular Weight	The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human BST2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BST2 Antibody, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA. determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human BST2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BST2 Antibody, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA. determined by ELISA.
	The purity of Human BST2 is greater than 95% as determined by SEC-HPLC.
	Human BST2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.