Human BTLA Protein (BTL-HM401)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human BTLA, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA. The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Lys31-Ser150

Catalog: BTL-HM401

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Product Description Recombinant Human BTLA Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Lys31-Ser150.
Product Category Recombinant Protein
Target BTLA
Synonyms CD272; BTLA; BTLA1; FLJ16065; MGC129743
Gene/Protein ID Q7Z6A9-1
Species Human
Exact Sequence Lys31-Ser150
Molecular Weight The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human BTLA, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA. The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200).
Immobilized Human BTLA, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA.
The purity of Human BTLA is greater than 95% as determined by SEC-HPLC.
Human BTLA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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