Human BTLA Protein (BTL-HM401)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Lys31-Ser150

Catalog: BTL-HM401

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Product Description	Recombinant Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150.
Product Category	Recombinant Protein

Target	BTLA
Synonyms	CD272; BTLA; BTLA1; FLJ16065; MGC129743
Gene/Protein ID	Q7Z6A9-1
Species	Human
Exact Sequence	Lys31-Ser150
Molecular Weight	The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200).
	Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test).
	Immobilized Human BTLA, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5ng/ml determined by ELISA.
	The purity of Human BTLA-is greater than 95% as determined by SEC-HPLC.
	Human BTLA-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.