Human BTLA Protein (BTL-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Lys31-Ser150

Catalog: BTL-HM401

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Product Description Recombinant Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150.

Product Background

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.

Product Category

Recombinant Protein

Target

BTLA
Synonyms CD272; BTLA; BTLA1; FLJ16065; MGC129743

Gene/Protein ID

Q7Z6A9-1

Species

Human

Exact Sequence

Lys31-Ser150
Molecular Weight The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200).
Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Immobilized Human BTLA, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5ng/ml determined by ELISA.
The purity of Human BTLA-is greater than 95% as determined by SEC-HPLC.
Human BTLA-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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