Human BTLA Protein (BTL-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Lys31-Ser150
Catalog #BTL-HM401
Regular price $330.00
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Product Description

Recombinant Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150.

Product Background

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

BTLA

Synonyms

CD272; BTLA; BTLA1; FLJ16065; MGC129743

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Lys31-Ser150

Molecular Weight

The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200). Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200).
Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
Immobilized Human BTLA, His Tag at 2ug/ml (100ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test). Immobilized Human BTLA, His Tag at 2ug/ml (100ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).
Human BTLA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BTLA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BTLA-is greater than 95% as determined by SEC-HPLC. The purity of Human BTLA-is greater than 95% as determined by SEC-HPLC.

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