Human BTN3A1/CD277 Protein (BTN-HM0A1)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BTN3A1, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln30-Gly254
Catalog #BTN-HM0A1
Regular price $315.00
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Product Description
Recombinant Human BTN3A1/CD277 Protein is expressed from HEK293 without tag.It contains Gln30-Gly254.
Product Background
The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
Product Category
Recombinant Protein
Target
BTN3A1/CD277
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln30-Gly254
Molecular Weight
The protein has a predicted MW of 24.15 kDa. Due to glycosylation, the protein migrates to 26-32 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human BTN3A1, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
No Tag
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human BTN3A1, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA.
Human BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC.

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