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Human BTN3A1/CD277 Protein, Ultra Low Endotoxin (BTN-HM1A1-UL) Summary
Product Description |
Recombinant Human BTN3A1/CD277 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln30-Gly254. |
Product Background |
The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg). |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human BTN3A1/CD277 Protein, Ultra Low Endotoxin (BTN-HM1A1-UL) Specifications/Details
Protein |
BTN3A1/CD277 |
Synonyms |
CD277; BTN3A1; BTF5; BT3.1; BTN3.1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln30-Gly254 |
Molecular Weight |
The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. |
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Human BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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