Human BTN3A3/BTF3 Protein (BNT-HM1A3) Summary
Product Description |
Recombinant Human BTN3A3/BTF3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln30-Trp248. |
Product Background |
The butyrophilin (BTN) family has many members with diverse functions related to immunomodulation, initiation and progression of tumors. BTN3A3 belongs to the BTN family, and exploring its expression and correlation with the prognosis of non-small cell lung cancer (NSCLC) patients has great clinical significance.The expression of BTN3A3 in NSCLC tissues was significantly lower than in adjacent tissues, and patients with low expression of BTN3A3 had late clinical stages and lower degree of tumor differentiation. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human BTN3A3/BTF3 Protein (BNT-HM1A3) Specifications/Details
Protein |
BTN3A3/BTF3 |
Synonyms |
BTN3A3; BTF3; BTN3.3; BTF3butyrophilin 3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln30-Trp248 |
Molecular Weight |
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human BTN3A3/BTF3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human BTN3A3/BTF3 is greater than 95% as determined by SEC-HPLC. |
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