Product Description |
Recombinant Human c-MPL/Thrombopoietin R Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Trp491. |
Product Background |
The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45% of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML. |
Product Category |
Recombinant Protein |
Target |
c-MPL/Thrombopoietin R |
Synonyms |
TPO-R; CD110; MPL; c-Mpl; MPLV; K12 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln26-Trp491 |
Molecular Weight |
The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human c-MPL, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). The affinity constant of 25.54 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human c-MPL, His Tag immobilized on CM5 Chip can bind Human Thrombopoietin, His Tag with an affinity constant of 25.54 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human c-MPL, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). |
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Human c-MPL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human c-MPL is greater than 95% as determined by SEC-HPLC. |
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