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Human CA12/Carbonic anhydrase XII Protein, Ultra Low Endotoxin (CAS-HM212-UL) Summary
Product Description |
Recombinant Human CA12/Carbonic anhydrase XII Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ala25-Ser301. |
Product Background |
Carbonic anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Carbonic anhydrase XII (CA XII) is a key mediator of several signaling pathways that are involved in cancer development. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CA12/Carbonic anhydrase XII Protein, Ultra Low Endotoxin (CAS-HM212-UL) Specifications/Details
Protein |
CA12/Carbonic anhydrase XII |
Synonyms |
Carbonic anhydrase; CA12; CAXII; Carbonic anhydrase 12; CA-XII; Tumor antigen HOM-RCC-3.1.3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala25-Ser301 |
Molecular Weight |
The protein has a predicted MW of 57.06 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 25mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human CA12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CA12 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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