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Human CA14/Carbonic Anhydrase XIV Protein (CA4-HM214) Summary
Product Description |
Recombinant Human CA14/Carbonic Anhydrase XIV Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ala16-Met290. |
Product Background |
Carbonic anhydrase XIV (CA XIV), a transmembrane protein, highly expressed in the central nervous system, is difficult to recombinantly express and purify in large scale for the measurements of inhibitor binding and drug design. CA XIV belongs to the family of twelve catalytically active CA isoforms in the human body. Disorders in the expression of CA XIV cause serious diseases and CA XIV has been described as a possible drug target for the treatment of epilepsy, some retinopathies, and skin tumors. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CA14/Carbonic Anhydrase XIV Protein (CA4-HM214) Specifications/Details
Protein |
CA14/Carbonic Anhydrase XIV |
Synonyms |
Carbonic anhydrase 14; EC:4.2.1.1; CA-XIV |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala16-Met290 |
Molecular Weight |
The protein has a predicted MW of 56.76 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its esterase activity. The specific activity is >100 pmol/min/µg. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Carbonic Anhydrase XIV on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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