Size exclusion chromatography (SEC-HPLC) purity profile of recombinant protein showing a single sharp peak eluting at 10 minutes.
Human CA2/Carbonic anhydrase II Protein (CAS-HM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its esterase activity. The specific activity is > 400 pmol/min/ug.
  • Amino Acid Range: Ser2-Lys260
Catalog #CAS-HM102
Regular price $223.30
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Human CA2/Carbonic anhydrase II Protein (CAS-HM102) Summary

Product Description

Recombinant Human CA2/Carbonic anhydrase II Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser2-Lys260.

Product Background

Carbonic anhydrase II (CA II) is a zinc metalloenzyme that catalyzes the reversible interconversion of water and CO2 to bicarbonate and a proton. CA II is abundant in most cells, and plays a role in numerous processes including gas exchange, epithelial ion transport, respiration, extra- and intracellular pH control, and vascular regulation.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human CA2/Carbonic anhydrase II Protein (CAS-HM102) Specifications/Details

Protein
CA2/Carbonic anhydrase II
Synonyms
Carbonic anhydrase 2; CA-II; Car2; CA2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser2-Lys260
Molecular Weight
The protein has a predicted MW of 30.72 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Bis-Tris PAGE result.
Activity
Measured by its esterase activity. The specific activity is > 400 pmol/min/ug.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human CA2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CA2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CA2 is greater than 95% as determined by SEC-HPLC. The purity of Human CA2 is greater than 95% as determined by SEC-HPLC.
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