Human CA2/Carbonic anhydrase II Protein (CAS-HM102) Summary
Product Description |
Recombinant Human CA2/Carbonic anhydrase II Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser2-Lys260. |
Product Background |
Carbonic anhydrase II (CA II) is a zinc metalloenzyme that catalyzes the reversible interconversion of water and CO2 to bicarbonate and a proton. CA II is abundant in most cells, and plays a role in numerous processes including gas exchange, epithelial ion transport, respiration, extra- and intracellular pH control, and vascular regulation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human CA2/Carbonic anhydrase II Protein (CAS-HM102) Specifications/Details
Protein |
CA2/Carbonic anhydrase II |
Synonyms |
Carbonic anhydrase 2; CA-II; Car2; CA2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser2-Lys260 |
Molecular Weight |
The protein has a predicted MW of 30.72 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its esterase activity. The specific activity is > 400 pmol/min/ug. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed