• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CA9, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 0.8ng/ml determined by ELISA. See testing image for detail.Measured by its esterase activity. The specific activity is >80 pmol/min/ยตg.
  • Amino Acid Range: Gln138-Asp414
Catalog #CA9-HM102
Price
Regular price $330.00
Regular price Sale price $330.00
/
Size
Avantor VWR Fisher Scientific

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Human CA9/Carbonic Anhydrase IX Protein (CA9-HM102) Summary

Product Description

 Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln138-Asp414.

Product Background

 CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.

Product Category

 Recombinant Protein / Antibody discovery / ADC targets

Human CA9/Carbonic Anhydrase IX Protein (CA9-HM102) Specifications/Details

Protein
 CA9/Carbonic Anhydrase IX
Synonyms
 CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln138-Asp414
Molecular Weight
 The protein has a predicted MW of 31.71 kDa. Due to glycosylation, the protein migrates to 32-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CA9, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 0.8ng/ml determined by ELISA. See testing image for detail.Measured by its esterase activity. The specific activity is >80 pmol/min/ยตg.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80ยฐC.๏ผ› Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CA9, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 0.8ng/ml determined by ELISA. Immobilized Human CA9, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 0.8ng/ml determined by ELISA.
Human CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CA9 is greater than 95% as determined by SEC-HPLC. The purity of Human CA9 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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