Name: Human CA9/Carbonic Anhydrase IX Protein (CA9-HM201)
Brand: KactusBio
SKU: CA9-HM201-100UG
Price: 330.0 USD
Availability: InStock

Human CA9/Carbonic Anhydrase IX Protein (CA9-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CA9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CA9 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gln38-Asp414

Catalog #CA9-HM201

Size

100UG

500UG

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Product Description	Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gln38-Asp414.
Product Background	CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.
Product Category	Recombinant Protein

Target	CA9/Carbonic Anhydrase IX
Accession	Q16790
Species	Human
Biotinylated	no
Amino Acid Range	Gln38-Asp414
Molecular Weight	The protein has a predicted MW of 66.91 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CA9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CA9 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CA9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CA9 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).
	Human CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CA9 is greater than 95% as determined by SEC-HPLC.

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