Product Description |
Recombinant Human CALCA/CGRP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala26-Asn141. |
Product Background |
Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
CALCA/CGRP |
Synonyms |
Calcitonin; CCP; PDN-21; CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; PCT; Procalcitonin; Calcitonin 1; Katacalcin |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala26-Asn141 |
Molecular Weight |
The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human CALCA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CALCA is greater than 95% as determined by SEC-HPLC. |
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