Human CALCA/CGRP Protein (CAL-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Ala26-Asn141

Catalog: CAL-HM201

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Product Description Recombinant Human CALCA/CGRP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala26-Asn141.

Product Background

 Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.

Product Category

 Recombinant Protein

Target

 CALCA/CGRP
Synonyms Calcitonin; CCP; PDN-21; CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; PCT; Procalcitonin; Calcitonin 1; Katacalcin

Gene/Protein ID

 NP_001732.1

Species

 Human

Exact Sequence

 Ala26-Asn141
Molecular Weight The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.

Product Tag

 C-hFc

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human CALCA/CGRP is greater than 95% as determined by SEC-HPLC.
Human CALCA/CGRP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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