Size-exclusion chromatography (SEC) analysis of recombinant human calprotectin (S100A8/S100A9) protein, showing a primary peak at ~8.65 minutes with minor impurities, indicating protein purity and aggregation status.
Human Calprotectin (S100A8&S100A9) Protein (CAL-HE101)
  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Met1-Glu93(S100A8) & Thr2-Pro114(S100A9)
Catalog #CAL-HE101
Regular price $375.00
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Human Calprotectin (S100A8&S100A9) Protein (CAL-HE101) Summary

Product Description

Recombinant Human Calprotectin (S100A8&S100A9) Protein is expressed from E.coli with His tag at the C-Terminus of S100A8.It contains Met1-Glu93(S100A8) and Thr2-Pro114(S100A9).

Product Background

Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human Calprotectin (S100A8&S100A9) Protein (CAL-HE101) Specifications/Details

Protein
Calprotectin (S100A8&S100A9)
Synonyms
Protein S100-A8;Calgranulin-A;CFAG;MRP-8;S100 calcium-binding protein A8;Protein S100-A9;MRP-14;S100 calcium-binding protein A9
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Met1-Glu93(S100A8) & Thr2-Pro114(S100A9)
Molecular Weight
The protein has a predicted MW of 11.7 kDa (S100A8) and 13.2 kDa (S100A9) same as Bis-Tris PAGE result.
Product Tag
C-His
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 2mM DTT, PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Calprotectin (S100A8&S100A9) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Calprotectin (S100A8&S100A9) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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