Human Calprotectin (S100A8&S100A9) Protein (CAL-HE101) Summary
Product Description |
Recombinant Human Calprotectin (S100A8&S100A9) Protein is expressed from E.coli with His tag at the C-Terminus of S100A8.It contains Met1-Glu93(S100A8) and Thr2-Pro114(S100A9). |
Product Background |
Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Calprotectin (S100A8&S100A9) Protein (CAL-HE101) Specifications/Details
Protein |
Calprotectin (S100A8&S100A9) |
Synonyms |
Protein S100-A8;Calgranulin-A;CFAG;MRP-8;S100 calcium-binding protein A8;Protein S100-A9;MRP-14;S100 calcium-binding protein A9 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Glu93(S100A8) & Thr2-Pro114(S100A9) |
Molecular Weight |
The protein has a predicted MW of 11.7 kDa (S100A8) and 13.2 kDa (S100A9) same as Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 2mM DTT, PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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