Product Description |
Recombinant Human Calprotectin (S100A8&S100A9) Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Glu93(S100A8)&Thr2-Pro114(S100A9). |
Product Background |
Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease. |
Product Category |
Recombinant Protein |
Target |
Calprotectin (S100A8&S100A9) |
Synonyms |
Protein S100-A8;Calgranulin-A;CFAG;MRP-8;S100 calcium-binding protein A8;Protein S100-A9;MRP-14;S100 calcium-binding protein A9 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Glu93(S100A8) & Thr2-Pro114(S100A9) |
Molecular Weight |
The protein has a predicted MW of 11.7 kDa(S100A8)&13.2 kDa(S100A9) same as Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 2mM DTT, PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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