- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Measured by its esterase activity. The specific activity is > 40 pmol/min/µg.
- Amino Acid Range: Ala25-Ser301
-
Other Available Proteins:
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Human CA12/Carbonic anhydrase XII Protein (CAS-HM112) Summary
Product Description |
Recombinant Human CA12/Carbonic anhydrase XII Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Ser301. |
Product Background |
Carbonic anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Carbonic anhydrase XII (CA XII) is a key mediator of several signaling pathways that are involved in cancer development. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human CA12/Carbonic anhydrase XII Protein (CAS-HM112) Specifications/Details
Protein |
CA12/Carbonic anhydrase XII |
Synonyms |
Carbonic anhydrase; CA12; CAXII; Carbonic anhydrase 12; CA-XII; Tumor antigen HOM-RCC-3.1.3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala25-Ser301 |
Molecular Weight |
The protein has a predicted MW of 32.22 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its esterase activity. The specific activity is > 40 pmol/min/µg. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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