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Human CCL24 Protein, Ultra Low Endotoxin (CCL-HM124-UL) Summary
Product Description |
Recombinant Human CCL24 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Val27-Cys119. |
Product Background |
C-C motif chemokine ligand 24 (CCL24) is a chemokine that regulates inflammatory and fibrotic activities through its receptor, C-C motif chemokine receptor (CCR3). CCL24 is a chemokine that regulates inflammation and fibrosis. It was found to be significantly expressed in patients with non-alcoholic steatohepatitis, in whom it regulates profibrotic processes in the liver. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CCL24 Protein, Ultra Low Endotoxin (CCL-HM124-UL) Specifications/Details
Protein |
CCL24 |
Synonyms |
CK-beta-6; Eotaxin-2; MPIF-2; MPIF2; SCYA24; Ckb-6; CCL24; member 24;CK-β-6 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val27-Cys119 |
Molecular Weight |
The protein has a predicted MW of 11.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CCL24, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CCL24 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA (QC Test). |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl, 100mM L-arginine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CCL24, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CCL24 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA (QC Test). |
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Human CCL24 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-CCL24 Antibody, hFc Tag on ProA-Biosensor can bind Human CCL24, His Tag with an affinity constant of 22.0 nM as determined in BLI assay. |
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The purity of Human CCL24 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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