Product Description |
Recombinant Human CCL5 Protein is expressed from E.coli with His-SUMO tag at the N-Terminus.It contains Ser24-Ser91. |
Product Background |
The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers. |
Product Category |
Recombinant Protein |
Target |
CCL5 |
Synonyms |
MuRantes; SIS-delta; Scya5; Ccl5; D17S136E; eoCP; RANTES; SCYA5; SISd; TCP228; CCL5 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser24-Ser91 |
Molecular Weight |
The protein has a predicted MW of 21 kDa. Due to the protein can cleaved into two chains, so it migrates to 15-18 kDa and 22-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His,SUMO |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris,0.3M NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). |
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Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA. |
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Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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